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Zebrafish help unlock mystery of motor neurone disease

28 Nov 2018

  • Breakthrough will accelerate research and drug trials to help tackle motor neurone disease (MND)
  • Trialling 1000 drugs on mice models would take more than 10 years, while trialling on zebrafish would only take a couple of months
  • Problems in the C9orf72 gene is the most common genetic cause of MND and frontotemporal dementia (FTD)

Scientists from the University of Sheffield have successfully created zebrafish that carry the complex genetic change known to cause the most common genetic form of motor neurone disease (MND).

The breakthrough will help to accelerate pioneering research and experimental drug trials to tackle the degenerative disease.

Until now, research to better understand how the disease occurs and the trial of experimental drugs has been conducted on fruit flies or mice models. This has had limited success due to the difference between the human brain and the fruit fly brain, and the time and cost implications of using mice models.

For the first time, researchers from the University of Sheffield’s Institute of Translational Neuroscience (SITraN) have successfully created the complex aspects of human C9-ALS/FTD pathobiology in zebrafish models.

This pioneering breakthrough is essential for studying the underlying mechanisms of MND and frontotemporal dementia (FTD).

MND,  also known as Amyotrophic Lateral Sclerosis (ALS), is a devastating neurogenerative disorder that affects the nerves – motor neurones – in the brain and spinal cord that tell your muscles what to do.

The messages from these nerves gradually stop reaching the muscles, leading them to weaken, stiffen and eventually waste. The progressive disease affects a patient’s ability to walk, talk, eat and breathe. MND affects 5,000 adults in the UK and there is currently no cure.

Approximately 10 per cent of MND cases are inherited but the remaining 90 per cent of MND cases are caused by complex genetic and environmental interactions which are currently not well understood – this is known as sporadic MND.

The most common known genetic cause of MND and FTD is a hexanucleotide expansion within the first intron of the C9orf72 gene. In this gene there are hundreds and thousands of repetitions of the sequence GGGGCC in patients with MND. This mutation is the largest genetic cause of MND and also the most predominant form of sporadic ALS.

Dr Tennore Ramesh, from SITraN at the University of Sheffield, said: “Using zebrafish models for MND research means that we can accelerate studies and our understanding of the devastating disease and other neurological conditions.

“Because zebrafish are transparent you can record results of studies much quicker and easier – the research is much less invasive.

“Trialing 1,000 drugs on mice models would take more than 10 years, however trialling 1,000 drugs on zebrafish would take only a couple of months.

“This will enable us to accelerate research into clinical trials in humans quicker than ever before.”

The four year project, which was led by Dr Ramesh in collaboration with leading researchers from SITraN including Professor Dame Pamela Shaw, Vice-President and Head of the Faculty of Medicine, Dentisry and Health at the University of Sheffield, is published in the journal Acta Neuropathologica Communications. The study was funded by the MND Association.

To view the full paper please visit: https://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0629-7

Additional Information

The University of Sheffield

With almost 29,000 of the brightest students from over 140 countries, learning alongside over 1,200 of the best academics from across the globe, the University of Sheffield is one of the world’s leading universities.

A member of the UK’s prestigious Russell Group of leading research-led institutions, Sheffield offers world-class teaching and research excellence across a wide range of disciplines.

Unified by the power of discovery and understanding, staff and students at the university are committed to finding new ways to transform the world we live in.

Sheffield is the only university to feature in The Sunday Times 100 Best Not-For-Profit Organisations to Work For 2018 and for the last eight years has been ranked in the top five UK universities for Student Satisfaction by Times Higher Education.

Sheffield has six Nobel Prize winners among former staff and students and its alumni go on to hold positions of great responsibility and influence all over the world, making significant contributions in their chosen fields.

Global research partners and clients include Boeing, Rolls-Royce, Unilever, AstraZeneca, Glaxo SmithKline, Siemens and Airbus, as well as many UK and overseas government agencies and charitable foundations.

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